A Review of Application of Proteomicsin the World's Biggest Killer Diseases and Some Foods

Ana Barati,Azadeh Khiabani, Mehran Hamzayeva

Proteomics, Biggest killer diseases, Malaria, Covid-19, Food, Proteomics

The proteome is defined as all the proteins expressed by the genome, an organism or a cell type. Proteomics is the study of the proteome of a cell line, tissue or organism with the aim of integrating the search for biological processes. The term proteomics refers to a wide range of studies related to the isolation of proteins from a single sample and the comparison of protein expression profiles in different samples. The knowledge of proteomics identifies the properties, quality and quantity of proteins. In the past years, various methods have been developed for proteomic analysis. One of the main goals in proteomic studies is to identify the desired proteins through their amino acid sequence. Whole proteome analysis is a major challenge. The most commonly used methods are two-dimensional gel electrophoresis (2DE), surface-enhanced laser desorption/ionization (SELDI), liquid chromatography-mass spectrometry (LC-MS), selective reaction monitoring (SRM), monitoring Multiplex reaction (MRM) and protein arrays The first step in the proteomic analysis is the isolation of proteins in samples. Two-dimensional gel electrophoresis is one of the most common separation methods in this field, and isoelectric focusing (IEF) in the first dimension and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) in the second dimension enables the separation of proteins by charge and size. Laser ionization and release methods by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) enable rapid ionization and identification of proteins and peptides. This article aims to comprehensively review proteomics in the worldof 's biggest killer diseases such as malaria, covid-19, Cancer and the some food items.

https://doi.org/10.31871/WJRR.16.1.5