A Review Study of Stability Indicating Based On RP-HPLC for Perindopril-Amlodipine Besylate

Vimal Kumar Sharma, Dr. U S Sharma, Devendra Singh Chahar

An accurate and precise HPLC method was developed for the simultaneous determination of perindopril and amlodipine. Separation of the drugs was achieved on a reverse phase C 18 column using mobile phase consisting of phosphate buffer 0.04M Borate cradle pH 3.0 the detection wavelength was 293nm & 339nm. The linearity was observed in the range of 10-90 μg/mL for amlodipine and 5-30 μg/mL for perindopril. The proposed method was validated for its linearity, accuracy, precision and robustness. The proposed method can be employed to estimate the drug contents in marketed formulations. Moreover the % RSD estimation of Intraday as well as Inter day investigation are 0.7026 as well as 0.1175 for Amlodipine besylate, 1.1703 as well as 1.1783 for Perindopril separately. Methods: This review paper will focus on Amlodipine A simple, precise and accurate RP-HPLC method has been developed and subsequently validated for simultaneous estimation of Perindopril–Amlodipine Besylate from their combination dosage form.