Contribution of Agricultural Practices on Enzymatic Activity in Anopheles Gambiae Populations from Cotton Areas in Northern Benin

Anges YADOULETON, Jean-Robert KLOTOE, Christophe CHABI

To have an idea on the contribution of agricultural practices on biochemical characterization of insecticide resistance in Anopheles gambiae populations, Anopheles larvae were collected from cotton field from July to September 2017 and reared until F1 generation. Enzymatic mechanisms (Glutathione-S-Transferase, esterase, monooxygenase P450 and total protein) were investigated from the F1 (the parental population after breeding) populations of An. gambiae s.l. Results from this study showed a significant high level of GST and monooxygenase P450 activities from the wild populations of An. gambiae from Banikoara compared to susceptible Kisumu strain (P>0.05). However, there is no significant difference in the level activity of Esterase (α and β-Naphthyl) from the wild populations of An. gambiae from compared to the susceptible Kisumu strain (P>0,05). These findings confirmed the contribution of agricultural practices on the selection of enzymatic activity in An. gambiae populations particularly on Glutathione-S-Transferase, and monooxygenase P450. However, the same level of the wild populations in esterase showed compared to the susceptible Kisumu strain showed that there is no clear link between the use of insecticide and enzymatic activity in An. gambiae populations.